HRP

Horseradish Peroxidase (HRP) is useful for many bioassays and has been used for electron microscopy as a transgene. HRP has the ability to catalyze the transfer of two electrons from a substrate to hydrogen peroxide via a two-electron oxidation of HRP event to generate water and an oxidized substrate. HRP is a popular detection label used for protein target detection, and is the most popular enzyme used in western blotting. It is also commonly used in immunohistochemistry and ELISA because it generates colored compounds. In photometric assays, HRP reacts with a substrate to produce chemiluminescent, chromogenic, or fluorescent signals upon oxidation, and it can amplify a weak signal from a target molecule.

HRP has several advantages over other reporters due to its combination of beneficial traits. HRP is robust, inexpensive, relatively small (allowing for more effective penetration into cells without risking interference with the conjugated protein function), readily binds to antibodies in an active form, oxidizes a wide range of substrates to produce optical signals, and more. The other key feature about HRP is that it has a high rate of turnover and this results in an efficient reaction, producing an abundance of reactant products in a short span of time at normal pH.

Note: Osprey Bio’s HRP does not have a subcellular localization signal.

Osprey Bio’s research indicated that HRP was one of the top used reporter genes in gene & cell therapy research due to its high sensitivity, simplicity, and wide usage applications.